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The most cited biological Institute in Russia *

Department of photosynthesis and fluorescent methods of investigation

Department was organized in 1976. Prof. A.Yu. Borisov was the first Head of Department. Since 1996 to present day Prof. A.P Razjivin is at the head.

Research areas

Theoretical and experimental studies of primary processes of photosynthesis. Further development and application of optical spectroscopy methods (derivative spectroscopy, fluorescence spectroscopy) in Life Sciences.

Major research advances

Demonstration of picosecond-scale processes of energy transfer from light-harvesting chlorophyll to reaction centers in purple and green bacteria and plant photosystem I  [Borisov AY, Godik VI. Fluorescence lifetime of bacteriochlorophyll and reaction center photooxidation in purple bacterium. Biochim Biophys Acta. 1970; 221: 441-3; Borisov AY, Godik VI. Energy transfer in bacterial photosynthesis. I. Light intensity dependences of fluorescence lifetimes. Bioenergetics. 1972; 3: 211-20; Borisov AY, Il’ina MD. Fluorescence lifetime and energy migration mechanism in photosystem-1 of plants. Biochim Biophys Acta. 1973; 305: 364-73; Borisov AY, Fetisova ZG, Godik VI. Energy transfer in photoactive complexes obtained from green bacterium Ch. limicola. Biochim Biophys Acta. 1977; 461: 501-9]. Discovery of "minor" form signal in chromatophores from purple bacteria [Razjivin AP et al. The study of excitation transfer between light-harvesting antenna and reaction center in chromatophores from purple bacterium Rhodospirillum rubrum by selective picosecond spectroscopy. FEBS Lett. 1982; 143: 40-4; Borisov AY et al. Minor component B-905 of light-harvesting antenna in Rhodospirillum rubrum chromatophores and the mechanism of singlet-singlet annihilation as studied by difference selective picosecond spectroscopy. FEBS Lett. 1982; 138: 25-8]. Development of polaron mechanism of energy conversion in photosynthetic reaction centers [Fok MV, Borisov AY. Water and energy conversion in photosynthesis. Studia biophysica. 1981; 84: 115-24; Borisov AY. New concept of energy migration and trapping in purple bacteria. Biochem Mol Biol Int. 1995; 35: 833-40]. Development of exciton theory of primary processes of photosynthesis (excitation energy transfer and trapping by means of excitations delocalized over circular aggregates of bacteriochlorophyll molecules in light-harvesting antenna of photosynthetic bacteria [Novoderezhkin VI, Razjivin AP. Excitonic interactions in the light-harvesting antenna of photosynthetic purple bacteria and their influence on picosecond absorbance spectra. FEBS Lett. 1993; 330: 5-7; Novoderezhkin VI, Razjivin AP. Exciton dynamics in circular aggregates: Application to antenna of photosynthetic purple bacteria. Biophys J. 1995; 68: 1089-1100; Novoderezhkin VI, Razjivin AP. The theory of Forster-type migration between clusters of strongly interacting molecules: application to light-harvesting complexes of purple bacteria. Chem Physics. 1996; 211: 203-214; Dracheva TV, Novoderezhkin VI, Razjivin AP. Exciton delocalization in the light-harvesting LH2 complex of photosynthetic purple bacteria. Photochem Photobiol. 1997; 66: 141-6].

Grants and projects

Grants of Russian Foundation of Basic Research: "Interrelation of spectral and structural characteristics of photosynthetic antennae according experimental and theoretical investigations" (A.P.Razjivin, 2003-2005); "Excitation transfer in pigment-protein complexes of photosynthesis: experimental investigations by non-linear spectroscopy and theoretical simulation" (A.Yu. Borisov, 2005-2007); Russian-Dutch grant RFBR-NWO "Mathematical modeling of energy transfer in photosynthesis" (V.I. Novoderezhkin, 2004-2006), Interdisciplinary project of Moscow State University "Biosensors on the basis of new fluorescent proteins: directed synthesis and optical properties" (A.P. Razjivin,  2004-2005). In preceding years - grants from INTAS, WTZ, G.Soros's International Science Foundation, Dutch NWO as well as several grants from RFBR.


I.Shuvalov's avard of Moscow State University (V.I.Novoderezhkin, 000).

Recent papers
  1. Omarova E.O., Nazarov P.A., Firsov A.M., Strakhovskaya M.G., Arkhipova A.Y., Moisenovich M.M., Agapov I.I., Ol'shevskaya V.A., Zaitsev A.V., Kalinin V.N., Kotova E.A., Antonenko Y.N. (2015) Carboranyl-Chlorin e(6) as a Potent Antimicrobial Photosensitizer. PLoS One, 10 (11): . >>

  2. Gall A., Ilioaia C., Kruger T.P.J, Novoderezhkin V.I., Robert B., Van Grondelle R. (2015) Conformational Switching in a Light-Harvesting Protein as Followed by Single-Molecule Spectroscopy. Biophys. J., 108 (11): 2713-2720. >>

  3. Rokitskaya T.I., Firsov A.M., Kotova E.A., Antonenko Y.N. (2015) Photodynamic Inactivation of Gramicidin Channels in Bilayer Lipid Membranes: Protective Efficacy of Singlet Oxygen Quenchers Depends on Photosensitizer Location. Biochem.-Moscow, 80 (6): 745-751. >>

  4. Antonenko Y.N., Nechaeva N.L., Baksheeva V.E., Rokitskaya T.I., Plotnikov E.Y., Kotova E.A., Zorov D.B. (2015) Intramitochondrial accumulation of cationic Atto520-biotin proceeds via voltage-dependent slow permeation through lipid membrane. Biochim. Biophys. Acta-Biomembr., 1848 (6): 1277-1284. >>

  5. Firsov A.M., Kotova E.A., Korepanova E.A., Osipov A.N., Antonenko Y.N. (2015) Peroxidative permeabilization of liposomes induced by cytochrome c/cardiolipin complex. Biochim. Biophys. Acta-Biomembr., 1848 (3): 767-774. >>

  6. Romero E., Augulis R., Novoderezhkin V.I., Ferretti M., Thieme J., Zigmantas D., Van Grondelle R. (2014) Quantum coherence in photosynthesis for efficient solar-energy conversion. Nat. Phys., 10 (9): 677-683. >>

  7. Denisov S.S., Kotova E.A., Khailova L.S., Korshunova G.A., Antonenko Y.N. (2014) Tuning the hydrophobicity overcomes unfavorable deprotonation making octylamino-substituted 7-nitrobenz-2-oxa-1,3-diazole (n-octylamino-NBD) a protonophore and uncoupler of oxidative phosphorylation in mitochondria. Bioelectrochemistry, 98: 30-38. >>

  8. Rokitskaya T.I., Kotova E.A., Agapov I.I., Moisenovich M.M., Antonenko Y.N. (2014) Unsaturated lipids protect the integral membrane peptide gramicidin A from singlet oxygen. FEBS Lett., 588 (9): 1590-1595. >>

  9. Taisova A.S., Yakovlev A.G., Fetisova Z.G. (2014) Size variability of the unit building block of peripheral light-harvesting antennas as a strategy for effective functioning of antennas of variable size that is controlled in vivo by light intensity. Biochem.-Moscow, 79 (3): 251-259. >>

  10. Antonenko Y.N., Kotova E.A., Omarova E.O., Rokitskaya T.I., Ol'Shevskaya V.A., Kalinin V.N., Nikitina R.G., Osipchuk J.S., Kaplan M.A., Ramonova A.A., Moisenovich M.M., Agapov I.I., Kirpichnikov M.P. (2014) Photodynamic activity of the boronated chlorin e6 amide in artificial and cellular membranes. Biochim. Biophys. Acta-Biomembr., 1838 (3): 793-801. >>

  11. Shchepinova M.M., Denisov S.S., Kotova E.A., Khailova L.S., Knorre D.A., Korshunova G.A., Tashlitsky V.N., Severin F.F., Antonenko Y.N. (2014) Dodecyl and octyl esters of fluorescein as protonophores and uncouplers of oxidative phosphorylation in mitochondria at submicromolar concentrations. Biochim. Biophys. Acta-Bioenerg., 1837 (1): 149-158. >>

  12. Curutchet C., Novoderezhkin V.I., Kongsted J., Munoz-Losa A. Van Grondelle R., Scholes G.D., Mennucci B. (2013) Energy Flow in the Cryptophyte PE545 Antenna Is Directed by Bilin Pigment Conformation. J. Phys. Chem. B, 117 (16): 4263-4273. >>

  13. Antonenko Y.N., Khailova L.S., Knorre D.A., Markova O.V., Rokitskaya T.I., Ilyasova T.M., Severina I.I., Kotova E.A., Karavaeva Y.E., Prikhodko A.S., Severin F.F., Skulachev V.P. (2013) Penetrating Cations Enhance Uncoupling Activity of Anionic Protonophores in Mitochondria. PLoS One, 8 (4): . >>

  14. Sorochkina A.I., Plotnikov E.Y., Rokitskaya T.I., Kovalchuk S.I., Kotova E.A., Sychev S.V., Zorov D.B., Antonenko Y.N. (2012) N-Terminally Glutamate-Substituted Analogue of Gramicidin A as Protonophore and Selective Mitochondrial Uncoupler. PLoS One, 7 (7): . >>

  15. Taisova A.S., Lukashev E.P., Fedorova N.V., Zobova A.V., Dolgova T.A., Fetisova Z.G. (2012) Experimental Proof of Optimality of Interfacing of Chlorosome BChl c and Membrane BChl a Subantennae in Superantenna of Photosynthetic Green Bacteria from the Oscillochloridaceae Family. Dokl. Biochem. Biophys., 444 (1): 154-157. >>

  16. Rokitskaya T.I., Sorochkina A.I., Kovalchuk S.I., Egorova N.S., Kotova E.A., Sychev S.V., Antonenko Y.N. (2012) The pH-dependent induction of lipid membrane ionic permeability by N-terminally lysine-substituted analogs of gramicidin A. Eur. Biophys. J. Biophys. Lett., 41 (2): 129-138. >>

  17. Sytina O.A., Novoderezhkin V.I., Van Grondelle R., Groot M.L. (2011) Modeling of Multi-Exciton Transient Absorption Spectra of Protochlorophyllide Aggregates in Aqueous Solution. Journal of Physical Chemistry A, 115 (43): 11944-11951.

  18. Kotova E.A., Kuzevanov A.V., Pashkovskaya A.A., Antonenko Y.N. (2011) Selective permeabilization of lipid membranes by photodynamic action via formation of hydrophobic defects or pre-pores. Biochimica et Biophysica Acta-Biomembranes, 1808 (9): 2252-2257.

  19. Stuart T.A.C., Vengris M., Novoderezhkin V.I., Cogdell R.J., Hunter C.N., Van Grondelle R. (2011) Direct Visualization of Exciton Reequilibration in the LH1 and LH2 Complexes of Rhodobacter sphaeroides by Multipulse Spectroscopy. Biophysical Journal, 100 (9): 2226-2233.

  20. Perevoshchikova I.V., Kotova E.A., Antonenko Y.N. (2011) Fluorescence correlation spectroscopy in biology, chemistry, and medicine. Biochemistry-Moscow, 76 (5): 497-516.

  21. Novoderezhkin V.I., Stuart T.A.C., Van Grondelle R. (2011) Dynamics of Exciton Relaxation in LH2 Antenna Probed by Multipulse Nonlinear Spectroscopy. Journal of Physical Chemistry A, 115 (16): 3834-3844.

  22. Rokitskaya T.I., Kolodkin N.I., Kotova E.A., Antonenko Y.N. (2011) Indolicidin action on membrane permeability: Carrier mechanism versus pore formation. Biochimica et Biophysica Acta-Biomembranes, 1808 (1): 91-97.

  23. Zobova A., Taisova A.S., Lukashev E.P., Fedorova N., Baratova L., Fetisova Z.G. (2011) CsmA Protein is Associated with BChl a in the Baseplate Subantenna of Chlorosomes of the Photosynthetic Green Filamentous BacteriumOscillochloris trichoides belonging to the Family Oscillochloridaceae. Journal of Biophysics, 2011: 10. >>

  24. Rokitskaya T.I., Macrae M.X., Blake S., Egorova N.S., Kotova E.A., Yang J., Antonenko Y.N. (2010) Mechanistic insight into gramicidin-based detection of protein-ligand interactions via sensitized photoinactivation. Journal of Physics-Condensed Matter, 22 (45): -.

  25. Moisenovich M.M., ol'shevskaya V.A., Rokitskaya T.I., Ramonova A.A., Nikitina R.G., Savchenko A.N., Tatarskiy V.V., Kaplan M.A., Kalinin V.N., Kotova E.A., Uvarov O.V., Agapov I.I., Antonenko Y.N., Shtil A.A. (2010) Novel Photosensitizers Trigger Rapid Death of Malignant Human Cells and Rodent Tumor Transplants via Lipid Photodamage and Membrane Permeabilization. Plos One, 5 (9): -.

  26. Zobova A.V., Taisova A.S., Fetisova Z.G. (2010) Search for an optimal interfacing of subantennae in superantenna of photosynthetic green bacteria from Oscillochloridaceae family: Model calculations. Doklady Biochemistry and Biophysics, 433 (1): 148-151.

  27. Novoderezhkin V.I., Doust A.B., Curutchet C., Scholes G.D., Van Grondelle R. (2010) Excitation Dynamics in Phycoerythrin 545: Modeling of Steady-State Spectra and Transient Absorption with Modified Redfield Theory. Biophysical Journal, 99 (2): 344-352.

  28. Perevoshchikova I.V., Zorov S.D., Kotova E.A., Zorov D.B., Antonenko Y.N. (2010) Flux of fluorescently labeled ATP through mitochondrial outer membrane can be regulated by hexokinase binding. Biochimica et Biophysica Acta-Bioenergetics, 1797: 68.

  29. Kruger T.P.J., Novoderezhkin V.I., Ilioaia C., Van Grondelle R. (2010) Fluorescence Spectral Dynamics of Single LHCII Trimers. Biophysical Journal, 98 (12): 3093-3101.

  30. Perevoshchikova I.V., Zorov S.D., Kotova E.A., Zorov D.B., Antonenko Y.N. (2010) Hexokinase inhibits flux of fluorescently labeled ATP through mitochondrial outer membrane porin. FEBS Letters, 584 (11): 2397-2402.

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