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Department of Isotopic Analysis

Department of Isotopic Analysis

Division was found in 1965. Prof. V. Fedoseev was first head of the division (1965-1966). Then division was led by D.Chem.Sc. A. Tarasenko (1966-1968), PhD E.Senchenkov (1968-1978), D.Ch.Sc. I. Kozlov (1978-1985). Since 1985 till nowadays department of Isotopic Analysis is headed by PhD Galina Ya. Kolomiytseva.

Research areas

Department of isotopic analysis is a service division, it supports researches of scientific divisions of the Institute in biochemistry and molecular biology. The division help becomes inevitable when radioactive substances and novel radiation measuring techniques (from low-energy β-radiators like ³Н, ¹⁴С, ³²Р, ³³Р, ³⁵S etc.) are essential. Unique instruments and acquisition systems developed by department's staff make measurements the radiation from complicated biological objects and macromolecules possible.
Own research work of the Department is linked to investigation of chromatin structure and its modification under effect of different chemical and physical agents. Important features of apoptosis process in the model plant system were described as well as the role of H1 histone in epigenetic regulation of major processes in the cell.

Research results

Physicochemical properties of desoxyribonucleoproteid which mimic DNA-histone interaction were described in different environmental conditions. DNA-protein contacts in the chromatin were studied and some of them were identified with the help of cross-linking agents (UV-light, γ-radiation and heavy metals). The behavior of preformed cross-links was observed after a cross-linking agent was removed. For the case of ionizing radiation it was shown that speed and efficiency of reparation of DNA crosslinks depend on tissue kind, stage of cell cycle and radiation properties.

Novel phenomenon was discovered: decrease in amount of DNA-protein crosslinks when mice were exposed to both Zn²⁺-cations and chronic γ-radiation in comparison to effect of distinct agents. Nonlinear kind of modifying of different biochemical characteristics under prolonged chronic radiation (model of low-dose effect), in several evidences of hormesis-effect was found.

Structure of chromatin and RNA-polimerase II content were determined in Physarum polycephalum culture in exponential phase of growth at different stages of cell cycle. Naturally synchronous culture appeared to demonstrate specific phenomenology having traits of apoptosis.

Recent studies focus at behavior of H1 histone upon apoptotic aging of сoleoptiles of wheat seeds; specific features of this object as compared with animals were described.

Grants and projects

Own scientific researches of the department were supported by RFBR.

Prizes

Advances of the Department were marked with awards of GKNT (USSR), Minvuz (USSR) and different research contests (scientific and methodological).

Employees

Assistant employees

Galina Ya. Kolomiytseva senior researcher, PhD (Chemistry)

Selected papers Recent papers

Kolomiitseva G.Y., Prusov A.N., Smirnova T.A., Vanyushin B.F. (2010) Influence of distamycin A on the methylation, extraction, and formation of UV-inducible DNA-protein cross links of histone H1 in the interphase nuclei of rat liver cells. Russian Journal of Bioorganic Chemistry, 36 (5): 620-627.
Smirnova T.A., Prusov A.N., Kolomijtseva G.Y., Vanyushin B.F. (2004) H1 histone in developing and aging coleoptiles of etiolated wheat seedlings. Biochemistry-Moscow, 69 (10): 1128-1135.
Grigoryev M.V., Kolomijtseva G.Y., Puchkov P.V. (1999) DNA-protein cross-links in cells of internal organs of rats after fractional irradiation at various doses. Biochemistry-Moscow, 64 (2): 204-206.
Kurochkina L., Kolomijtseva G. (1992) Photoinduced Cross-Linking of Histones-H3 and Histones-H1 to DNA in Deoxyribonucleoprotein - Implication in Studying Histone-Dna Interactions. Biochemical and Biophysical Research Communications, 187 (1): 261-267.

Prusov A.N., Smirnova T.A., Kolomijtseva G.Y. (2015) Extraction of histone H1 and decondensation of nuclear chromatin with various Mg-dependent organization levels under treatment with polyglutamic acid and distamycin. Biochem.-Moscow, 80 (3): 356-365. >>
Fedoreyeva L.I., Smirnova T.A., Kolomijtseva G.Y., Vanyushin B.F. (2013) Modulation of action of wheat seedling endonucleases WEN1 and WEN2 by histones. Biochem.-Moscow, 78 (5): 505-516. >>
Fedoreyeva L.I., Smirnova T.A., Kolomijtseva G.Y., Khavinson V.K., Vanyushin B.F. (2013) Interaction of short peptides with FITC-labeled wheat histones and their complexes with deoxyribooligonucleotides. Biochem.-Moscow, 78 (2): 166-175. >>
Prusov A.N., Smirnova T.A., Kolomijtseva G.Y. (2013) Influence of chromatin structure, antibiotics, and endogenous histone methylation on phosphorylation of histones H1 and H3 in the presence of protein kinase A in rat liver nuclei in vitro. Biochem.-Moscow, 78 (2): 176-184. >>
Muntyan M.S., Morozov D.A., Klishin S.S., Khitrin N.V., Kolomijtseva G.Y. (2012) Evaluation of the electrical potential on the membrane of the extremely alkaliphilic bacterium Thioalkalivibrio. Biochem.-Moscow, 77 (8): 917-924. >>
Mikhailova A.G., Khairullin R.F., Kolomijtseva G.Y., Rumsh L.D. (2012) Oligopeptidase B from Serratia proteamaculans. III. Inhibition analysis. Specific interactions with metalloproteinase inhibitors. Biochem.-Moscow, 77 (3): 300-306. >>
Prusov A.N., Smirnova T.A., Kurochkina L.P., Kolomijtseva G.Y. (2010) Influence of Distamycin, Chromomycin, and UV-irradiation on Extraction of Histone H1 from Rat Liver Nuclei by Polyglutamic Acid. Biochemistry-Moscow, 75 (11): 1331-1341.
Kolomiitseva G.Y., Prusov A.N., Smirnova T.A., Vanyushin B.F. (2010) Influence of distamycin A on the methylation, extraction, and formation of UV-inducible DNA-protein cross links of histone H1 in the interphase nuclei of rat liver cells. Russian Journal of Bioorganic Chemistry, 36 (5): 620-627.
Fedoreyeva L.I., Smirnova T.A., Kolomijtseva G.Y., Vanyushin B.F. (2009) H1 histone modulates DNA hydrolysis with WEN1 and WEN2 endonucleases from wheat coleoptiles. Biochemistry-Moscow, 74 (2): 145-152.
Smirnova T.A., Kolomiitseva G.Y., Prusov A.N., Vanyushin B.F. (2006) Zinc and copper content in developing and aging Coleoptiles of wheat seedlings. Russian Journal of Plant Physiology, 53 (4): 535-540.
Zyryanov A.B., Tammenkoski M., Salminen A., Kolomiytseva G.Y., Fabrichniy I.P., Goldman A., Lahti R., Baykov A.A. (2004) Site-specific effects of zinc on the activity of family II pyrophosphatase. Biochemistry, 43 (45): 14395-14402.
Smirnova T.A., Prusov A.N., Kolomijtseva G.Y., Vanyushin B.F. (2004) H1 histone in developing and aging coleoptiles of etiolated wheat seedlings. Biochemistry-Moscow, 69 (10): 1128-1135.
Dunaevskii Y.E., Aleksandrushkina N.I., Smirnova T.A., Kolomiitseva G.Y., Vanyushin B.F., Belozersky M.A. (2003) Effect of antioxidant BHT on the proteolytic apparatus of aging coleoptiles of wheat seedlings grown in light. Russian Journal of Bioorganic Chemistry, 29 (5): 459-463.
Osipov A.N., Ryabchenko N.I., Ivannik B.P., Dzikovskaya L.A., Ryabchenko V.I., Kolomijtseva G.Y. (2003) A prior administration of heavy metals reduces thymus lymphocyte DNA lesions and lipid peroxidation in gamma-irradiated mice. Journal de Physique Iv, 107: 987-992.
Grigoryev M.V., Kolomijtseva G.Y., Puchkov P.V. (1999) DNA-protein cross-links in cells of internal organs of rats after fractional irradiation at various doses. Biochemistry-Moscow, 64 (2): 204-206.
Osipov A.N., Sypin V.D., Kolomijtseva G.Y. (1999) DNA-protein cross-links in different organs of mice induced by the combined action of zinc and gamma-irradiation. Biochemistry-Moscow, 64 (2): 201-203.
Osipov A.N., Sypin V.D., Kolomijtseva G.Y., Polsky O.G., Ilyinov A.N. (1997) DNA-protein cross-links in leukocytes of mice induced by Zn, Cd, and Pb. Biochemistry-Moscow, 62 (6): 681-683.
Prusov A.N., Kolomijtseva G.Y. (1997) Effect of UV-irradiation of rat liver nuclei on structural transitions and fractionation of the chromatin. Biochemistry-Moscow, 62 (6): 667-675.
Kurochkina L.P., Kolomijtseva G.Y. (1996) UV-Cross-Linking of the globular domain of histone H1 with DNA in deoxyribonucleoprotein. Biochemistry-Moscow, 61 (6): 757-763.
Osipov A.N., Kolomijtseva G.Y. (1996) Post-irradiation change of DNA-protein cross-links and single-strand DNA breaks in different organs of gamma-irradiated rats. Biochemistry-Moscow, 61 (5): 667-670.
Kolomijtseva G.Y., Kurochkina L.P., Bobkova E.A. (1994) Study of Ultraviolet Radiation-Induced DNA-Protein Cross-Links in Hela-Cells. Biochemistry-Moscow, 59 (8): 891-894.
Kurochkina L., Kolomijtseva G. (1992) Photoinduced Cross-Linking of Histones-H3 and Histones-H1 to DNA in Deoxyribonucleoprotein - Implication in Studying Histone-Dna Interactions. Biochemical and Biophysical Research Communications, 187 (1): 261-267.
Kurochkina L.P., Kolomijtseva G.Y. (1989) Isolation of Modified Histone-H3 from Ultraviolet-Irradiated Deoxyribonucleoprotein by Reversed-Phase High-Performance Liquid-Chromatography. Analytical Biochemistry, 178 (1): 88-92.