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Самый цитируемый биологический институт РФ *

Швядас Витаутас-Юозапас Каятоно

зав.отделом, профессор, доктор биологических наук
Статьи
  1. Shcherbakova T.A., Panin N.V., Suplatov D.A., Shapovalova I.V., Svedas V.K. (2015) The beta D484N mutant of penicillin acylase from Escherichia coli is more resistant to inactivation by substrates and can effectively perform peptide synthesis in aqueous medium. J. Mol. Catal. B-Enzym., 112: 66-68. >>

  2. Grigorenko B.L., Khrenova M.G., Nilov D.K., Nemukhin A.V., Svedas V.K. (2014) Catalytic Cycle of Penicillin Acylase from Escherichia coli: QM/MM Modeling of Chemical Transformations in the Enzyme Active Site upon Penicillin G Hydrolysis. ACS Catal., 4 (8): 2521-2529. >>

  3. Novikov F.N., Stroganov O.V., Khaliullin I.G., Panin N.V., Shapovalova I.V., Chilov G.G., Svedas V.K. (2013) Molecular modeling of different substrate-binding modes and their role in penicillin acylase catalysis. FEBS J., 280 (1): 115-126. >>

  4. Suplatov D.A., Besenmatter W., Svedas V.K., Svendsen A. (2012) Bioinformatic analysis of alpha/beta-hydrolase fold enzymes reveals subfamily-specific positions responsible for discrimination of amidase and lipase activities. Protein Eng. Des. Sel., 25 (11): 689-697. >>

  5. Romanova N.N., Rybalko I.I., Tallo T.G., Zyk N.V., Svedas V.K. (2012) Synthesis of Schiff bases from 3-amino-3-arylpropionic acid esters in aqueous medium. Russ. J. Organ. Chem., 48 (6): 860-863. >>

  6. Guranda D.T., Ushakov G.A., Yolkin P.G., Svedas V.K. (2012) Thermodynamics of phenylacetamides synthesis: Linear free energy relationship with the pK of amine. J. Mol. Catal. B-Enzym., 74 (1): 48-53. >>

  7. Zakharenko A.L., Sukhanova M.V., Khodyreva S.N., Novikov F.N., Stroylov V.S., Nilov D.K., Chilov G.G., Svedas V.K., Lavrik O.I. (2011) Improved procedure of the search for poly(ADP-Ribose) polymerase-1 potential inhibitors with the use of the molecular docking approach. Molecular Biology, 45 (3): 517-521.

  8. Nilov D.K., Shabalin I.G., Popov V.O., Svedas V.K. (2011) Investigation of Formate Transport through the Substrate Channel of Formate Dehydrogenase by Steered Molecular Dynamics Simulations. Biochemistry-Moscow, 76 (2): 172-174.

  9. Chernorizov K.A., Elkina J.L., Semenyuk P.I., Svedas V.K., Muronetz V.I. (2010) Novel Inhibitors of Glyceraldehyde-3-phosphate Dehydrogenase: Covalent Modification of NAD-Binding Site by Aromatic Thiols. Biochemistry-Moscow, 75 (12): 1444-1449.

  10. Gardossi L., Poulsen P.B., Ballesteros A., Hult K., Svedas V.K., Vasic-racki D., Carrea G., Magnusson A., Schmid A., Wohlgemuth R., Halling P.J. (2010) Guidelines for reporting of biocatalytic reactions. Trends in Biotechnology, 28 (4): 171-180.

  11. Pchelintsev N.A., Youshko M.I., Svedas V.K. (2009) Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase. Journal of Molecular Catalysis B-Enzymatic, 56 (4): 202-207.

  12. Grinberg V.Y., Burova T.V., Grinberg N.V., Shcherbakova T.A., Guranda D.T., Chilov G.G., Svedas V.K. (2008) Thermodynamic and kinetic stability of penicillin acylase from Escherichia coli. Biochimica et Biophysica Acta-Proteins and Proteomics, 1784 (5): 736-746.

  13. Chernobrovkin M.G., Shapovalova E.N., Guranda D.T., Kudryavtsev P.A., Svedas V.K., Shpigun O.A. (2007) Chiral high-performance liquid chromatography analysis of alpha-amino acid mixtures using a novel SH reagent-N-R-mandelyl-L-cysteine and traditional enantiomeric thiols for precolumn derivatization. Journal of Chromatography A, 1175 (1): 89-95.

  14. Chilov G.G., Sidorova A.V., Svedas V.K. (2007) Quantum chemical studies of the catalytic mechanism of N-terminal nucleophile hydrolase. Biochemistry-Moscow, 72 (5): 495-500.

  15. Pchelintsev N.A., Youshko M.I., Svedas V.K. (2006) A new method for spectrophotometric assay of activity of cross-linked penicillin acylase aggregates. Biochemistry-Moscow, 71 (3): 315-319.

  16. Guranda D.T., Kudryavtsev P.A., Khimiuk A.Y., Svedas V.K. (2005) Efficient enantiomeric analysis of primary amines and amino alcohols by high-performance liquid chromatography with precolumn derivatization using novel chiral SH-reagent N-(R)-mandelyl-(S)-cysteine. Journal of Chromatography A, 1095 (1): 89-93.

  17. Pertsovich S.I., Guranda D.T., Podchernyaev D.A., Yanenko A.S., Svedas V.K. (2005) Aliphatic amidase from Rhodococcus rhodochrous M8 is related to the nitrilase/cyanide hydratase family. Biochemistry-Moscow, 70 (11): 1280-1287.

  18. Guranda D.T., Volovik T.S., Svedas V.K. (2004) pH stability of penicillin acylase from Escherichia coli. Biochemistry-Moscow, 69 (12): 1386-1390.

  19. Shcherbakova T.A., Korennykh A.V., Van Langen L.M., Sheldon R.A., Svedas V.K. (2004) Use of high acyl donor concentrations leads to penicillin acylase inactivation in the course of peptide synthesis. Journal of Molecular Catalysis B-Enzymatic, 31 (1): 63-65.

  20. Guranda D.T., Khimiuk A.I., Van Langen L.M., Van Rantwijk F., Sheldon R.A., Svedas V.K. (2004) An 'easy-on, easy-off' protecting group for the enzymatic resolution of (+/-)-1-phenylethylamine in an aqueous medium. Tetrahedron-Asymmetry, 15 (18): 2901-2906.

  21. Guranda D.T., Shapovalova I.V., Svedas V.K. (2004) A new N-acyl derivative of (S)-cysteine for quantitative determination of enantiomers of amino compounds by HPLC with a precolumn modification with o-phthalaldehyde. Russian Journal of Bioorganic Chemistry, 30 (5): 403-408.

  22. Youshko M.I., Van Langen L.M., Sheldon R.A., Svedas V.K. (2004) Application of aminoacylase I to the enantioselective resolution of alpha-amino acid esters and amides. Tetrahedron-Asymmetry, 15 (12): 1933-1936.

  23. Youshko M.I., Moody H.M., Bukhanov A.L., Boosten W.H.J., Svedas V.K. (2004) Penicillin acylase-catalyzed synthesis of beta-lactam antibiotics in highly condensed aqueous systems: Beneficial impact of kinetic substrate supersaturation. Biotechnology and Bioengineering, 85 (3): 323-329.

  24. Khimiuk A.Y., Korennykh A.V., Van Langen L.M., Van Rantwijk F., Sheldon R.A., Svedas V.K. (2003) Penicillin acylase-catalyzed peptide synthesis in aqueous medium: a chemo-enzymatic route to stereoisomerically pure diketopiperazines. Tetrahedron-Asymmetry, 14 (20): 3123-3128.

  25. Chilov G.G., Moody H.M., Boesten W.H.J., Svedas V.K. (2003) Resolution of (RS)-phenylglycinonitrile by penicillin acylase-catalyzed acylation in aqueous medium. Tetrahedron-Asymmetry, 14 (17): 2613-2617.

  26. Stroganov O.V., Chilov G.G., Svedas V.K. (2003) Force field parametrization for 6-aminopenicillanic acid. Journal of Molecular Structure-Theochem, 631: 117-125.

  27. Youshko M.I., Bukhanov A.L., Svedas V.K. (2003) Study of nucleophile binding in the penicillin acylase active center. Kinetic analysis. Biochemistry-Moscow, 68 (3): 334-338.

  28. Youshko M.I., Chilov G.G., Shcherbakova T.A., Svedas V.K. (2002) Quantitative characterization of the nucleophile reactivity in penicillin acylase-catalyzed acyl transfer reactions. Biochimica et Biophysica Acta-Proteins and Proteomics, 1599 (1): 134-140.

  29. Youshko M.I., Svedas V.K. (2002) Penicillin acylase-catalyzed solid-state ampicillin synthesis. Catalysis, 344 (8): 894-898.

  30. Van Langen L.M., Janssen M.H.A., Oosthoek N.H.P., Pereira S.R.M., Svedas V.K., Van Rantwijk F., Sheldon R.A. (2002) Active site titration as a tool for the evaluation of immobilization procedures of penicillin acylase. Biotechnology and Bioengineering, 79 (2): 224-228.

  31. Youshko M.I., Van Langen L.M., de Vroom E., Van Rantwijk F., Sheldon R.A., Svedas V.K. (2002) Penicillin acylase-catalyzed ampicillin synthesis using a pH gradient: A new approach to optimization. Biotechnology and Bioengineering, 78 (5): 589-593.

  32. Chilov G.G., Svedas V.K. (2002) Enzymatic hydrolysis of beta-lactam antibiotics at low pH in a two-phase "aqueous solution water-immiscible organic solvent" system. Canadian Journal of Chemistry-Revue Canadienne de Chimie, 80 (6): 699-707.

  33. Guranda D.T., Van Langen L.M., Van Rantwijk F., Sheldon R.A., Svedas V.K. (2001) Highly efficient and enantioselective enzymatic acylation of amines in aqueous medium. Tetrahedron-Asymmetry, 12 (11): 1645-1650.

  34. Youshko M.I., Van Langen L.M., de Vroom E., Van Rantwijk F., Sheldon R.A., Svedas V.K. (2001) Highly efficient synthesis of ampicillin in an "aqueous solution-precipitate" system: Repetitive addition of substrates in a semicontinuous process. Biotechnology and Bioengineering, 73 (5): 426-430.

  35. Youshko M.I., Svedas V.K. (2000) Kinetics of ampicillin synthesis catalyzed by penicillin acylase from E-coli in homogeneous and heterogeneous systems. Quantitative characterization of nucleophile reactivity and mathematical modeling of the process. Biochemistry-Moscow, 65 (12): 1367-1375.

  36. Van Langen L.M., Oosthoek N.H.P., Guranda D.T., Van Rantwijk F., Svedas V.K., Sheldon R.A. (2000) Penicillin acylase-catalyzed resolution of amines in aqueous organic solvents. Tetrahedron-Asymmetry, 11 (22): 4593-4600.

  37. Youshko M.I., Van Langen L.M., de Vroom E., Moody H.M., Van Rantwijk F., Sheldon R.A., Svedas V.K. (2000) Penicillin acylase-catalyzed synthesis of ampicillin in "aqueous solution-precipitate" systems. High substrate concentration and supersaturation effect. Journal of Molecular Catalysis B-Enzymatic, 10 (5): 509-515.

  38. Shamolina T.A., Svedas V.K. (2000) Reactivation of heterodimer and individual subunits of penicillin acylase from E-coli after inactivation in urea. Biochemistry-Moscow, 65 (6): 672-676.

  39. Van Langen L.M., Van Rantwijk F., Svedas V.K., Sheldon R.A. (2000) Penicillin acylase-catalyzed peptide synthesis: a chemo-enzymatic route to stereoisomers of 3,6-diphenylpiperazine-2,5-dione. Tetrahedron-Asymmetry, 11 (5): 1077-1083.

  40. Shamolina T.A., Drachev V.A., Svedas V.K. (1999) Activity of penicillin acylase from E-coli in the reversed-micelle system AOT-H2O-octane. Biochemistry-Moscow, 64 (10): 1186-1195.

  41. Youshko M.I., Sinev A.V., Svedas V.K. (1999) Stability and catalytic properties of penicillin acylase in systems with low water content. Biochemistry-Moscow, 64 (10): 1196-1199.

  42. Youshko M.I., Shamolina T.A., Guranda D.F., Synev A.V., Svedas V.K. (1998) Specific substrates for spectrophotometric determination of penicillin acylase activity. Biochemistry-Moscow, 63 (9): 1104-1109.

  43. Soloshonok V.A., Soloshonok I.V., Kukhar V.P., Svedas V.K. (1998) Biomimetic transamination of alpha-alkyl beta-keto carboxylic esters. Chemoenzymatic approach to the stereochemically defined alpha-alkyl beta-fluoroalkyl beta-amino acids. Journal of Organic Chemistry, 63 (6): 1878-1884.

  44. Svedas V.K., Beltser A.I. (1998) Totally enzymatic synthesis of peptides - Penicillin acylase-catalyzed protection and deprotection of amino groups as important building blocks of this strategy. Enzyme Engineering Xiv, 864: 524-527.

  45. Gurentsova O.I., Savchenko M.V., Sumbatyan N.V., Korshunova G.A., Svedas V.K. (1997) Detection of enantiomers of nucleoamino acids by HPLC with premodification with o-phthalic aldehyde. Bioorganicheskaya Khimiya, 23 (11): 877-881.

  46. Elsliger M.A., Pshezhetsky A.V., Vinogradova M.V., Svedas V.K., Potier M. (1996) Comparative modeling of substrate binding in the S-1' subsite of serine carboxypeptidases from yeast, wheat, and human. Biochemistry, 35 (47): 14899-14909.

  47. Svedas V.K., Savchenko M.V., Beltser A.I., Guranda D.F. (1996) Enantioselective penicillin acylase-catalyzed reactions - Factors governing substrate and stereospecificity of the enzyme. Enzyme Engineering Xiii, 799: 659-669.

  48. Soloshonok V.A., Fokina N.A., Rybakova A.V., Shishkina I.P., Galushko S.V., Sorochinsky A.E., Kukhar V.P., Savchenko M.V., Svedas V.K. (1995) Biocatalytic Approach to Enantiomerically Pure Beta-Amino Acids. Tetrahedron-Asymmetry, 6 (7): 1601-1610.

  49. Soloshonok V.A., Kirilenko A.G., Fokina N.A., Kukhar V.P., Galushko S.V., Svedas V.K., Resnati G. (1994) Chemoenzymatic Approach to the Synthesis of Each of the 4 Isomers of Alpha-Alkyl-Beta-Fluoroalkyl-Substituted Beta-Amino Acids. Tetrahedron-Asymmetry, 5 (7): 1225-1228.

  50. Soloshonok V.A., Kirilenko A.G., Fokina N.A., Shishkina I.P., Galushko S.V., Kukhar V.P., Svedas V.K., Kozlova E.V. (1994) Biocatalytic Resolution of Beta-Fluoroalkyl-Beta-Amino Acids. Tetrahedron-Asymmetry, 5 (6): 1119-1126.

  51. Solodenko V.A., Belik M.Y., Galushko S.V., Kukhar V.P., Kozlova E.V., Mironenko D.A., Svedas V.K. (1993) Enzymatic Preparation of Both L-Enantiomer and D-Enantiomer of Phosphonic and Phosphonous Analogs of Alanine Using Penicillin Acylase. Tetrahedron-Asymmetry, 4 (9): 1965-1968.

  52. Soloshonok V.A., Svedas V.K., Kukhar V.P., Kirilenko A.G., Rybakova A.V., Solodenko V.A., Fokina N.A., Kogut O.V., Galaev I.Y., Kozlova E.V., Shishkina I.P., Galushko S.V. (1993) An Enzymatic Entry to Enantiopure Beta-Amino Acids. Synlett, (5): 339-341.

  53. Kukhar V.P., Soloshonok V.A., Svedas V.K., Kotik N.V., Galaev I.Y., Kirilenko A.G., Kozlova E.V. (1993) Homochiral Organoelement Analogs of Natural Compounds .2. 3-Amino-4,4,4-Trifluorobutanoic Acid - Synthesis, Enzymatic Resolution and Determination of Absolute-Configurations of Enantiomers. Bioorganicheskaya Khimiya, 19 (4): 474-477.

  54. Soloshonok V.A., Galaev I.Y., Svedas V.K., Kozlova E.V., Kotik N.V., Shishkina I.P., Galushko S.V., Rozhenko A.B., Kukhar V.P. (1993) Homochiral Organoelement Analogs of Natural Compounds .1. Biocatalytic Methods for Production of Fluorine-Containing L-Phenylglycine and D-Phenylglycine on Preparative-Scale. Bioorganicheskaya Khimiya, 19 (4): 467-473.

  55. Soloshonok V.A., Svedas V.K., Kukhar V.P., Galaev I.Y., Kozlova E.V., Svistunova N.Y. (1993) Homochiral Organoelement Analogs of Natural Compounds .3. Biocatalytic Method for Production of Fluorosubstituted (R)-Phenylalanines and (S)-Phenylalanines, (S, R)-Phenylserines and (R, S)-Phenylserines. Bioorganicheskaya Khimiya, 19 (4): 478-483.

  56. Pshezhetsky A.V., Danilova R.A., Fedorova I.M., Sagimbaeva S.K., Pervushina S.V., Obuchova M.A., Svedas V.K., Ashmarin I.P. (1993) Influence of the Immunization Against Heterologous Alcohol-Dehydrogenase on Liver Alcohol-Dehydrogenase Isozymes and Alcohol-Abuse of Rats. European Journal of Biochemistry, 212 (3): 757-761.

  57. Petrauskas A.A., Svedas V.K. (1991) Hydrophobicity of Beta-Lactam Antibiotics - Explanation and Prediction of Their Behavior in Various Partitioning Solvent Systems and Reversed-Phase Chromatography. Journal of Chromatography, 585 (1): 3-34.

  58. Petrauskas A.A., Svedas V.K. (1991) Hydrophobicity of Beta-Lactam Antibiotics - Explanation and Prediction of Their Behavior in Various Partitioning Solvent Systems and Reversed-Phase Chromatography. Journal of Chromatography, 585 (1): 3-34.

  59. Solodenko V.A., Kasheva T.N., Kukhar V.P., Kozlova E.V., Mironenko D.A., Svedas V.K. (1991) Preparation of Optically-Active 1-Aminoalkylphosphonic Acids by Stereoselective Enzymatic-Hydrolysis of Racemic N-Acylated 1-Aminoalkylphosphonic Acids. Tetrahedron, 47 (24): 3989-3998.

  60. Didziapetris R.J., Svedas V.K. (1991) Penicillin Acylase-Catalyzed Acyl Group Transfer to Amino-Acids, Their Esters and Peptides - A Kinetic-Study. Biomedica Biochimica Acta, 50 (10): S237-S242.

  61. Gololobov M.Y., Schellenberger U., Schellenberger F., Jakubke H.D., Svedas V.K. (1990) Peptide-Synthesis Catalyzed by Proteases - Influence of Temperature on the Kinetics of Acyl Transfer Catalyzed by Papain. Biochemistry-Moscow, 55 (2): 251-256.

  62. Gololobov M.Y., Schellenberger U., Schellenberger F., Jakubke H.D., Svedas V.K. (1990) Peptide-Synthesis Catalyzed by Proteases - Influence of Temperature on the Kinetics of Acyl Transfer Catalyzed by Papain. Biochemistry-Moscow, 55 (2): 251-256.

  63. Gololobov M.Y., Borisov I.L., Belikov V.M., Svedas V.K. (1988) Acyl Group Transfer by Proteases Forming Acyl Enzyme Intermediate - Kinetic-Model Analysis. Biotechnology and Bioengineering, 32 (7): 866-872.

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